Journal of Plant Registrations
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Published in JOURNAL OF PLANT REGISTRATIONS 1:171-172 (2007)
DOI: 10.3198/jpr2006.09.0610crg
© 2007 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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GERMPLASMS

Registration of Near-Isogenic Hard Spring Wheat Lines Differing in Puroindoline Alleles

J. D. Shermana, S. P. Lanninga, D. Clarkb and L. E. Talberta,*

a Plant Sciences and Plant Pathology Dep., Montana State Univ., Bozeman MT 59717
b Westbred LLC, Bozeman MT 59717. Research was partially supported by USDA/CREES-IFAFS award 2001-04462 and USDA/CREES-NRICAP award 2006-55606-16629

* Corresponding author (usslt{at}montana.edu).

Montana State University announces the release of a set of near-isogenic lines: MAS 21-3 (Reg. No. GP-816, PI 643400), MAS 21-10 (Reg. No. GP-817, PI 643401), MAS 22-5 (Reg. No. GP-818, PI 643402), MAS 22-6 (Reg. No. GP-819, PI 643403), MAS 3G-BB (Reg. No. GP-820, PI 643404), MAS 3G-AA (Reg. No. GP-821, PI 643405), MAS 3B-BB (Reg. No. GP-822, PI 643406), MAS 3B-AA (Reg. No. GP-823, PI 643407), MAS 71-3 (Reg. No. GP-824, PI 643408), MAS 71-1 (Reg. No. GP-825, PI 643409), MAS 70-3 (Reg. No. GP-826, PI 643410), MAS 70-15 (Reg. No. GP-827, PI 643411), MAS 46-8 (Reg. No. GP-828, PI 643412), MAS 46-10 (Reg. No. GP-829, PI 643413), MAS 47-1 (Reg. No. GP-830, PI 643414), MAS 47-5 (Reg. No. GP-831, PI 643415), MAS 141-5 (Reg. No. GP-832, PI 643416), MAS 141-4 (Reg. No. GP-833, PI 643417), MAS 142-8 (Reg. No. GP-834, PI 643418), and MAS 142-7 (Reg. No. GP-835, PI 643419) (Table 1 ), varying for alleles at the Puroindoline (Pin) loci controlling grain hardness. The pairs of isogenic lines contain either a null mutation at Puroindoline a (Pina-D1b) or a point mutation at the tightly linked gene Puroindoline b (Pinb-D1b). Either mutation causes hard kernel texture in wheat (Giroux and Morris, 1998). Previous research has shown that Pina-D1a/Pinb-D1b genotypes are softer textured, produce higher flour yield, and produce flour that gives a higher loaf volume than Pina-D1b/Pinb-D1a genotypes (Martin et al., 2001). The isogenic lines developed in this study will provide experimental material to study the basis for texture and quality differences in hard wheats.


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  		Table 1. Near isogenic wheat lines varying for genotype at the Puroindoline locus.

 
The Pina-D1a/Pinb-D1b donor line was ‘Choteau’ hard red spring wheat (Lanning et al., 2004). Six backcrosses were made to hard red spring wheat cultivars with genotype Pina-D1b/PinB-D1a, including ‘McNeal’ (Lanning et al., 1994), ‘Scholar’ (Lanning et al., 2000), ‘Outlook’ (Lanning et al., 2003), and ‘Hank’. Hank was developed by Westbred LLC (Bozeman MT). Hard white spring wheat cultivar ‘Explorer’ with genotype Pina-D1b/Pinb-D1a (Lanning et al., 2002) was also used as a recurrent parent. Cultivars were used as the female parent in all generations. Beginning with the BC1 generations, progeny lines heterozygous for Pina-D1a/Pinb-D1b and Pina-D1b/Pinb-D1a were selected using previously described primer sets (Martin et al., 2001). After the final backcross, BC6F2 individuals were selected to be homozygous for Pina-D1a/Pinb-D1b and Pina-D1b/Pinb-D1a, respectively. Seed of the F3 generation was grown in the field in Bozeman MT in 2005, and F4 seed was tested for grain hardness using the Single Kernel Characterization System 4100 (Perten Instruments North America Incorporated, Springfield IL). Rows of F4 seed were grown in the field in Bozeman in 2006, and harvested F5 seed again tested for grain hardness. No selection was made for morphological or agronomic characteristics until field evaluation of F4 rows.

Three to six pairs of isogenic lines were tested for each recurrent parent. Mean hardness of the Pina-D1a/Pinb-D1b near-isogenic lines was less than their Pina-D1b/Pinb-D1a counterparts for each recurrent parent in 2005 based on two-tailed t tests (P < 0.05). In 2006, the differences among respective near-isogenic lines within a recurrent parent were all significant based on two-tailed t tests (P < 0.05), except that McNeal Pina-D1b/Pinb-Dla and McNeal Pina-D1a/Pinb-D1b lines were not significantly different (P = 0.10). No significant differences (P < 0.05) in grain protein were detected for near-isogenic lines for any of the recurrent parents. Table 1 shows pairs of near-isogenic lines selected for release based on hardness scores representative of the Pin genotype for that recurrent parent, and based on phenotypic performance in the field indistinguishable from the recurrent parent. Pairs of lines in the same family (Table 1) descend from a single F1 plant from the final backcross. Near-isogenic lines for recurrent parent Outlook were not available for testing in 2005, thus only 2006 hardness data are available.

Limited quantities of seeds (5 g) of each near-isogenic line are available on request from the corresponding author for five years. Recipients of seeds are asked to make appropriate recognition of the source of genetic stocks if they are used in the development of a new cultivar, germplasm, parental line, or hybrid.

Footnotes

All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.

Received for publication September 22, 2006.

References





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