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a Dep. of Crop and Soil Sciences
b Dep. of Plant Pathology, Univ. of Georgia, Athens, GA 30602
c USDA-ARS Soybean/Maize Germplasm, Urbana, IL 61801. Contribution from the Georgia Agric. Exp. Stn
* Corresponding author (rboerma{at}uga.edu).
Six soybean [Glycine max (L.) Merr] germplasm lines were developed by the Georgia Agricultural Experiment Stations and released in 2005: G95-Has339 (Reg. No. 344, PI 644054), G95-Has551 (Reg. No. 345, PI 644055), G95-Has1452 (Reg. No. 346, PI 644056), G95-Has1536 (Reg. No. 347, PI 644057), G95-Has2539 (Reg. No. 348, PI 644058), and G95-Has4243 (Reg. No. 349, PI 644059). They were selected within the productive soybean cultivar ‘Haskell’ (Boerma et al., 1994) with differences in seed protein, seed oil, seed weight, plant height, lodging, or maturity. These lines have utility as parents to develop elite breeding populations or use in the study of genetic and physiological mechanisms responsible for conditioning the phenotypes of the selected variants within Haskell.
The six Haskell-derived germplasm lines were developed by growing single plants in 1995 from 1994 Haskell Foundation seed in a replicated-3 honeycomb design (Fasoulas and Fasoula, 1995). The honeycomb trial was planted in three-seeded hills with a spacing of 0.90 m between hills to eliminate the negative effect of interplant competition on selection efficiency (Fasoula and Fasoula, 1997, 2000; Fasoula and Tollenaar, 2005). Each hill was thinned to one plant per hill and the trial had the density of 1.4 plants m2. Plants were grown to maturity, harvested by hand, and threshed on site (Fasoula and Boerma, 2005). Seeds from each single plant were tested for chemical composition, and divergent selection of plants for high or low protein and oil content was performed (Fasoula and Boerma, 2005). In 1996, 40 lines derived from single plants contrasting most for protein or oil content, plus four entries of Haskell were planted in a three-replicate randomized complete block design near Athens, GA. Plots were one row 3.5 m long with 0.76 m between rows. Data recorded for each plot were maturity, seed weight, seed protein content, and seed oil content.
In 1997, the 32 most divergent lines for the various traits and four Haskell entries were grown in a three-replicate randomized complete block design near Athens and Plains, GA (Fasoula and Boerma, 2005, 2007). Plots were two rows 4 m long with 0.76 m between rows. Data were collected for seed yield, seed weight, seed protein and oil, maturity, lodging, and plant height. In 1998, the most divergent Haskell-derived lines for each trait were planted in a similar experiment near Athens and Plains, GA (Fasoula and Boerma, 2005, 2007). The experimental unit was the same as in 1997. Data were combined across years, and the six lines that were most divergent from Haskell either in seed protein, seed oil, seed weight, plant height, lodging, or maturity were selected for release. To provide a conservative test of significance (low probability of a Type I error) for the comparison of the Haskell-derived lines with Haskell, the line x environment interaction mean square was used as the error variance, and an LSD was calculated at the 
= 0.001 probability level (Table 1
).
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G95-Has551, G95-Has4243, G95-Has1536, G95-Has339, G95-Has1452, and G95-Has2539, like Haskell, have determinate growth habit, purple flowers, tawny pubescence, and tan pod walls with shiny yellow seed and black hila. Disease and nematode resistance of each line are similar to Haskell.
G95-Has551, G95-Has4243, G95-Has1536, G95-Has339, G95-Has1452, and G95-Has2539 will be maintained by the Dep. of Crop and Soil Sciences at the Univ. of Georgia, Athens, GA 30602. Small quantities of seed for research and breeding can be obtained from the corresponding author. Seeds of these lines have been deposited in the National Plant Germplasm System (NPGS) for research purposes, including development and commercialization of new cultivars. Appropriate recognition of the source should be noted if G95-Has551, G95-Has4243, G95-Has1536, G95-Has339, G95-Has1452, and G95-Has2539 contribute to the development of new genetic stocks, molecular tools, germplasm, or cultivars.
Footnotes
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Received for publication March 26, 2006.
References
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