Journal of Plant Registrations
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Published in JOURNAL OF PLANT REGISTRATIONS 1:158-159 (2007)
DOI: 10.3198/jpr2006.03.0199crg
© 2007 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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GERMPLASMS

Registration of Seven Soybean Germplasm Lines Selected within the Cultivar ‘Cook’ Differing in Seed and Agronomic Traits

Vasilia A. Fasoulaa, H. Roger Boermaa,*, Jennifer L. Yatesa, David R. Walkerc, Steven L. Finnertyb, Gina B. Rowana and E. Dale Wooda

a Dep. of Crop and Soil Sciences
b Dep. of Plant Pathology, Univ. of Georgia, Athens, GA 30602
c USDA-ARS Soybean/Maize Germplasm, Urbana, IL 61801. Contribution from the Georgia Agric. Exp. Stn

* Corresponding author (rboerma{at}uga.edu).

Seven soybean [Glycine max (L.) Merr] germplasm lines were developed by the Georgia Agricultural Experiment Stations and released in 2005: G95-Cook319 (Reg. No. GP-337, PI 644047), G95-Cook1346 (Reg. No. GP-338, PI 644048), G95-Cook2014 (Reg. No. GP-339, PI 644049), G95-Cook2734 (Reg. No. GP-340, PI 644050), G95-Cook3008 (Reg. No. GP-341, PI 644051), G95-Cook3614 (Reg. No. GP-342, PI 644052), and G95-Cook3746 (Reg. No. GP-343, PI 644053). They were selected within the productive soybean cultivar ‘Cook’ (Boerma et al., 1992) with differences in seed protein, seed oil, seed weight, plant height, or maturity. These lines have utility as parents to develop elite breeding populations or use in the study of genetic and physiological mechanisms responsible for conditioning the phenotypes of the selected variants within Cook.

The seven Cook-derived germplasm lines were developed by growing single plants in 1995 from 1994 Cook Foundation seed in a replicated-3 honeycomb design (Fasoulas and Fasoula, 1995). The honeycomb trial was planted in three-seeded hills with a spacing of 0.90 m between hills to eliminate plant competition and maximize the yield potential per plant (Fasoula and Fasoula, 1997, 2000; Fasoula and Tollenaar, 2005). Each hill was thinned to one plant per hill and the trial had the density of 1.4 plants/m–2 (Fasoula and Boerma, 2005). Plants were grown to maturity, harvested by hand, and threshed on site. Seed from each single plant was tested for chemical composition and divergent selection of plants for high or low protein and oil content was performed (Fasoula and Boerma, 2005). In 1996, 40 lines derived from single plants contrasting most for protein or oil content plus four entries of Cook were planted in a three-replicate randomized complete block design near Athens, GA. Plots were in one row 3.5 m long with 0.76 m between rows. Data recorded for each plot were maturity, seed weight, seed protein content, and seed oil content.

In 1997, the 32 most divergent lines for the various traits and the four Cook entries were grown in a three-replicate randomized complete block design near Athens and Plains, GA (Fasoula and Boerma, 2005, 2007). Plots were in two rows 4 m long with 0.76 m between rows. Data were collected for seed yield, seed weight, seed protein and oil, maturity, and plant height. In 1998, the most divergent Cook-derived lines for each trait were planted in a similar experiment near Athens and Plains, GA (Fasoula and Boerma, 2005, 2007). The experimental unit was the same as in 1997. Data were combined across years and the seven lines that were most divergent from Cook either in seed protein, seed oil, seed weight, plant height, or maturity were selected for release. To provide a conservative test of significance (low probability of a Type I error) for the comparison of the Cook-derived lines with Cook, the line x environment interaction mean square was used as the error variance, and an LSD was calculated at the {alpha} = 0.001 probability level (Table 1 ).


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Table 1. Mean seed composition and agronomic performance of seven intra-cultivar selections from Cook evaluated across years (Fasoula and Boerma 2005, 2007).

 
G95-Cook1346 averaged 11 g kg–1 higher seed protein (431 g kg–1) than Cook when tested in five environments across three years (Table 1). It was similar to Cook in seed oil, seed weight, maturity, plant height, and seed yield. G95-Cook3614 produced 10 g kg–1 more seed protein (430 g kg–1) than Cook and 11 mg seed–1 greater seed weight than Cook. It matured 2 d later, while similar to Cook in seed oil, plant height, and seed yield. G95-3746 averaged 9 g kg–1 higher seed protein (429 g kg–1) and 5 g kg–1 lower seed oil than Cook. It was similar to Cook in seed weight, maturity, plant height, and seed yield. G95-Cook3008 matured 2 d later than Cook, while it was similar to Cook in seed yield, plant height, and seed protein and seed oil content (Table 1). G95-Cook319 matured 2 d earlier than Cook and averaged 9 mg seed–1 lower seed weight across years. Seed protein and oil content, plant height, and seed yield were similar to those of Cook. G95-Cook2734 averaged 7 cm taller, while G95-Cook2014 averaged 8 cm shorter than Cook. Their seed protein and oil content, seed weight, maturity, and seed yield were similar to those of Cook.

G95-Cook1346, G95-Cook3614, G95-Cook3746, G95-Cook3008, G95-Cook319, G95-Cook2734, and G95-Cook2014, like Cook, have determinate growth habit, purple flowers, tawny pubescence, and tan pod walls with dull yellow seed and black hila. The intensity of the black pigment in the hilum can vary across environments and even on different seeds from the same plant. Disease and nematode resistance of each line is similar to Cook.

G95-Cook1346, G95-Cook3614, G95-Cook3746, G95-Cook3008, G95-Cook319, G95-Cook2734, and G95-Cook2014 will be maintained by the Dep. of Crop and Soil Sciences at the Univ. of Georgia, Athens, GA 30602. Small quantities of seeds for research and breeding can be obtained from the corresponding author. Seed of these lines has been deposited in the National Plant Germplasm System for research purposes, including development and commercialization of new cultivars. Appropriate recognition of the source should be noted if G95-Cook1346, G95-Cook3614, G95-Cook3746, G95-Cook3008, G95-Cook319, G95-Cook2734, or G95-Cook2014 contributes to the development of new genetic stocks, molecular tools, germplasm, or cultivars.

Footnotes

All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.

Received for publication March 26, 2006.

References





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