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GENETIC STOCK

Registration of NFLT12, a Tissue Culture–Responsive Lolium temulentum (Darnel Ryegrass) Line

Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73401

^* Corresponding author (zywang{at}noble.org).

ABSTRACT

Lolium temulentum L. (Darnel ryegrass) is closely related to major forage and turf species, is self-pollinated, diploid, and has a short life cycle. These traits make it suitable to serve as a model species for testing gene functions in grasses. A new L. temulentum line, NFLT12 (Reg. No. GS-7, PI 655941), was developed using anther culture on an F₂ population derived from a cross of two parental L. temulentum lines. Haploid and doubled haploid plants were obtained and their tissue culture responsiveness and amiability to genetic transformation were tested. NFLT12 is highly responsive to tissue culture, is readily transformable and is expected to provide a resource for those using L. temulentum as a model species for functional genomics studies.

Abbreviations: MS, Murashige and Skoog