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GERMPLASM

Registration of a Hexaploid Wheat Translocation Line Carrying a Short Segment of Chromosome 5A^m including Softness Genes Pina and Pinb from Triticum monococcum

^a Instituto de Recursos Biológicos, INTA Castelar (1686) Hurlingham, Buenos Aires, Argentina
^b Dep. of Plant Sciences, Univ. of California, Davis, CA 95616-8780. This project was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service (CSREES) grant number 2006-55606-16629, and by Argentinean grants BID 1728/OC-AR PID 234 and PICTO 08-12948

^* Corresponding author (jdubcovsky{at}ucdavis.edu).

ABSTRACT

Translocation line T5A^mS-5AS·5AL R#45 (Reg. No. GP-839, PI 651012) carries the distal region of Triticum monococcum L. chromosome 5A^mS translocated into the 5AS chromosome of T. aestivum L. cultivar Chinese Spring. This translocation line was developed in collaboration with the Instituto de Recursos Biológicos, INTA, Argentina, and the University of California, Davis, CA. The distal T. monococcum 5A^mS segment in T5A^mS-5AS·5AL R#45 is approximately 8 cM long and carries the active softness genes Pina and Pinb in the genetic background of Chinese Spring. This translocation replaced the deleted Pina and Pinb puroindoline genes from T. aestivum by the functional orthologs from T. monococcum, significantly reducing the hardness of the grain. Translocation line T5A^mS-5AS·5AL R#45 has a shorter segment of T. monococcum chromosome 5A^m relative to previous translocations, minimizing linkage drag and the probability of negative effects associated with the introgression of alien genes.

Abbreviations: CS, Chinese Spring